The Two-Component System ChtRS Contributes to Chlorhexidine Tolerance in Enterococcus faecium.

ABSTRACT: Enterococcus faecium is one of the primary causes of nosocomial infections. Disinfectants are commonly used to prevent infections with multidrug-resistant E. faecium in hospitals. Worryingly, E. faecium strains that exhibit tolerance to disinfectants have already been described. We aimed to identify and characterize E. faecium genes that contribute to tolerance to the disinfectant chlorhexidine (CHX). We used a transposon mutant library, constructed in a multidrug-resistant E. faecium bloodstream isolate, to perform a genome-wide screen to identify genetic determinants involved in tolerance to CHX. We identified a putative two-component system (2CS), composed of a putative sensor histidine kinase (ChtS) and a cognate DNA-binding response regulator (ChtR),

SUBMITTER: Guzman Prieto AM 

PROVIDER: S-EPMC5404517 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature