Microglia-derived IL-1β promotes chemokine expression by Muller cells and RPE in focal retinal degeneration.

ABSTRACT:

Background

Chemokine signalling is required for the homing of leukocytes during retinal inflammation, and is associated with pathogenesis of diseases such as age-related macular degeneration (AMD). Here, we explore the role of interleukin-1β (IL-1β) in modulating AMD-associated chemokines Ccl2, Cxcl1, and Cxcl10 during photo-oxidative retinal damage, and the effect on both the accumulation of outer-retinal macrophages, and death of photoreceptors.

Methods

Inhibition of retinal IL-1β expression was performed using either siRNA or antibody neutralisation, which was intravitreally injected in SD rats prior to photo-oxidative damage. Changes in the expression and localisation of Il-1β, Ccl2, Cxcl1 and Cxcl10 genes were assessed using qPCR and in situ hybridisation, while the rec

SUBMITTER: Natoli R 

PROVIDER: S-EPMC5404662 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature