Mass cytometry reveals a distinct immunoprofile of operational tolerance in pediatric liver transplantation.

ABSTRACT: Long-term IS in transplant patients has significant morbidity, poorer quality of life, and substantial economic costs. TOL, defined as graft acceptance without functional impairment in the absence of IS, has been achieved in some pediatric LT recipients. Using mass cytometry, peripheral blood immunotyping was performed to characterize differences between tolerant patients and patients who are stable on single-agent IS. Single-cell mass cytometry was performed using blood samples from a single-center pediatric LT population of operationally tolerant patients to comprehensively characterize the immune cell populations in the tolerant state compared with patients on chronic low-dose IS. Specific T-cell populations of interest were confirmed by flow cytometry. This high-dimensional phenotypic

SUBMITTER: Lau AH 

PROVIDER: S-EPMC5404744 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature