Hepatic Prominin-1 expression is associated with biliary fibrosis.

ABSTRACT:

Background

Intrahepatic biliary fibrosis, as seen with cholestatic liver injuries such as biliary atresia, is mechanistically distinct from fibrosis caused by hepatocyte toxicity. We previously demonstrated the expansion of cells expressing the stem/progenitor cell marker Prominin-1, within regions of developing fibrosis in biliary atresia. Thus, we hypothesized that Prominin-1 expression is biliary fibrosis-specific.

Methods

Gene expression of Prominin-1 was analyzed in adult mice undergoing either cholestatic bile duct ligation or hepatotoxic carbon tetrachloride administration by quantitative polymerase chair reaction. Lineage tracing of Prominin-1-expressing cells and Collagen-1α-expressing cells was performed after bile duct ligation in Prominin-1^{cre-ert2-lacz}

SUBMITTER: Nguyen MV 

PROVIDER: S-EPMC5404955 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature