Nonenzymatic glycation interferes with fibronectin-integrin interactions in vascular smooth muscle cells.

ABSTRACT:

Objective

We aimed to investigate whether advanced nonenzymatic glycation of the ECM protein, fibronectin, impacts its normal integrin-mediated interaction with arteriolar VSMC.

Methods

AFM was performed on cultured VSMC from rat cremaster arterioles to study native and glycated fibronectin (FN and gFN) interactions with cellular integrins. AFM probes were functionalized with FN or gFN or with native or glycated albumin (gAlb) as controls.

Results

VSMC showed increased adhesion probability to gFN (72.9±3.5%) compared with native FN (63.0±1.6%). VSMC similarly showed increased probability of adhesion (63.8±1.7%) to gAlb compared with native Alb (40.1±4.7%). Adhesion of native FN to VSMC was α5 and β1 integrin dependent whereas adhesion of gFN to VSMC was integrin indep

SUBMITTER: Dhar S 

PROVIDER: S-EPMC5404995 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature