Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice.

ABSTRACT:

Background

Spatial- and temporal-specific expression patterns are primarily regulated at the transcriptional level by gene promoters. Therefore, it is important to identify the binding motifs of transcription factors to better understand the networks associated with embryogenesis.

Results

Here, we used a protein-binding microarray (PBM) to identify the binding motifs of OsSMF1, which is a basic leucine zipper transcription factor involved in the regulation of rice seed maturation. OsSMF1 (previously called RISBZ1 or OsbZIP58) is known to interact with GCN4 motifs (TGA(G/C)TCA) to regulate seed storage protein synthesis, and it functions as a key regulator of starch synthesis. Quadruple 9-mer-based PBM analysis and electrophoretic mobility shift assay revealed that OsSMF1 bou

SUBMITTER: Kim JS 

PROVIDER: S-EPMC5405039 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature