Flow cytometry sorting of nuclei enables the first global characterization of Paramecium germline DNA and transposable elements.

ABSTRACT:

Background

DNA elimination is developmentally programmed in a wide variety of eukaryotes, including unicellular ciliates, and leads to the generation of distinct germline and somatic genomes. The ciliate Paramecium tetraurelia harbors two types of nuclei with different functions and genome structures. The transcriptionally inactive micronucleus contains the complete germline genome, while the somatic macronucleus contains a reduced genome streamlined for gene expression. During development of the somatic macronucleus, the germline genome undergoes massive and reproducible DNA elimination events. Availability of both the somatic and germline genomes is essential to examine the genome changes that occur during programmed DNA elimination and ultimately decipher the mechanisms underlyi

SUBMITTER: Guerin F 

PROVIDER: S-EPMC5405496 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature