MicroRNA-125a-3p is regulated by MyD88 in Legionella pneumophila infection and targets NTAN1.

ABSTRACT:

Background

Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. It is highly adapted to intracellular replication and manipulates host cell functions like vesicle trafficking and mRNA translation to its own advantage. However, it is still unknown to what extent microRNAs (miRNAs) are involved in the Legionella-host cell interaction.

Methods

WT and MyD88-/- murine bone marrow-derived macrophages (BMM) were infected with L. pneumophila, the transcriptome was analyzed by high throughput qPCR array (microRNAs) and conventional qPCR (mRNAs), and mRNA-miRNA interaction was validated by luciferase assays with 3´-UTR mutations and western blot.

Results

L. pneumophila infection caused a pro-inflammatory reaction and significant miRNA changes in mur

SUBMITTER: Jentho E 

PROVIDER: S-EPMC5406027 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature