Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae.

ABSTRACT:

Background

Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD⁺-dependent acetate reduction has been shown to improve ethanol yields and contribute to detoxification of acetate-containing media. However, the osmosensitivity of glycerol non-producing strains limits their applicability in high-osmolarity industrial processes. This study explores engineering strategies for minimizing glycerol production by acetate-reducing strains, while retaining osmotolerance.

Results

GPD2 encodes one of two S. cerevisiae isoenzymes of NAD⁺-dependent glycerol-3-p

SUBMITTER: Papapetridis I 

PROVIDER: S-EPMC5406903 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature