Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis.

ABSTRACT:

Background

Cancer/testis (CT) genes have expression normally restricted to the testis, but become activated during oncogenesis, so they have excellent potential as cancer-specific biomarkers. Evidence is starting to emerge to indicate that they also provide function(s) in the oncogenic programme. Human TEX19 is a recently identified CT gene, but a functional role for TEX19 in cancer has not yet been defined.

Methods

siRNA was used to deplete TEX19 levels in various cancer cell lines. This was extended using shRNA to deplete TEX19 in vivo. Western blotting, fluorescence activated cell sorting and immunofluorescence were used to study the effect of TEX19 depletion in cancer cells and to localize TEX19 in normal testis and cancer cells/tissues. RT-qPCR and RNA sequencing were e

SUBMITTER: Planells-Palop V 

PROVIDER: S-EPMC5406905 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature