Shear stress induces Gα_q/11 activation independently of G protein-coupled receptor activation in endothelial cells.

ABSTRACT: Mechanochemical signal transduction occurs when mechanical forces, such as fluid shear stress, are converted into biochemical responses within the cell. The molecular mechanisms by which endothelial cells (ECs) sense/transduce shear stress into biological signals, including the nature of the mechanosensor, are still unclear. G proteins and G protein-coupled receptors (GPCRs) have been postulated independently to mediate mechanotransduction. In this study, we used in situ proximity ligation assay (PLA) to investigate the role of a specific GPCR/Gα_q/11 pair in EC shear stress-induced mechanotransduction. We demonstrated that sphingosine 1-phosphate (S1P) stimulation causes a rapid dissociation at 0.5 min of Gα_q/11 from its receptor S1P₃, followed by an increa

SUBMITTER: Dela Paz NG 

PROVIDER: S-EPMC5407018 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature