Multiple Aggregation Pathways in Human γS-Crystallin and Its Aggregation-Prone G18V Variant.

ABSTRACT:

Purpose

Cataract results from the formation of light-scattering precipitates due to point mutations or accumulated damage in the structural crystallins of the eye lens. Although excised cataracts are predominantly amorphous, in vitro studies show that crystallins are capable of adopting a variety of morphologies depending on the preparation method. Here we characterize thermal, pH-dependent, and UV-irradiated aggregates from wild-type human γS-crystallin (γS-WT) and its aggregation-prone variant, γS-G18V.

Methods

Aggregates of γS-WT and γS-G18V were prepared under acidic, neutral, and basic pH conditions and held at 25°C or 37°C for 48 hours. UV-induced aggregates were produced by irradiation with a 355-nm laser. Aggregation and fibril formation were monitored via turbidity

SUBMITTER: Roskamp KW 

PROVIDER: S-EPMC5407245 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature