Engineering genetic circuit interactions within and between synthetic minimal cells.

ABSTRACT: Genetic circuits and reaction cascades are of great importance for synthetic biology, biochemistry and bioengineering. An open question is how to maximize the modularity of their design to enable the integration of different reaction networks and to optimize their scalability and flexibility. One option is encapsulation within liposomes, which enables chemical reactions to proceed in well-isolated environments. Here we adapt liposome encapsulation to enable the modular, controlled compartmentalization of genetic circuits and cascades. We demonstrate that it is possible to engineer genetic circuit-containing synthetic minimal cells (synells) to contain multiple-part genetic cascades, and that these cascades can be controlled by external signals as well as inter-liposomal communication witho

SUBMITTER: Adamala KP 

PROVIDER: S-EPMC5407321 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature