Intracellular Trafficking, Localization, and Mobilization of Platelet-Borne Thiol Isomerases.

ABSTRACT:

Objective

Thiol isomerases facilitate protein folding in the endoplasmic reticulum, and several of these enzymes, including protein disulfide isomerase and ERp57, are mobilized to the surface of activated platelets, where they influence platelet aggregation, blood coagulation, and thrombus formation. In this study, we examined the synthesis and trafficking of thiol isomerases in megakaryocytes, determined their subcellular localization in platelets, and identified the cellular events responsible for their movement to the platelet surface on activation.

Approach and results

Immunofluorescence microscopy imaging was used to localize protein disulfide isomerase and ERp57 in murine and human megakaryocytes at various developmental stages. Immunofluorescence microscopy and subcel

SUBMITTER: Crescente M 

PROVIDER: S-EPMC5407363 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature