Cloning and characterization of the Type I Baeyer-Villiger monooxygenase from Leptospira biflexa.

ABSTRACT: Baeyer-Villiger monooxygenases are recognized by their ability and high selectivity as oxidative biocatalysts for the generation of esters or lactones using ketones as starting materials. These enzymes represent valuable tools for biooxidative syntheses since they can catalyze reactions that otherwise involve strong oxidative reagents. In this work, we present a novel enzyme, the Type I Baeyer-Villiger monooxygenase from Leptospira biflexa. This protein is phylogenetically distant from other well-characterized BVMOs. In order to study this new enzyme, we cloned its gene, expressed it in Escherichia coli and characterized the substrate scope of the Baeyer-Villiger monooxygenase from L. biflexa as a whole-cell biocatalyst. For this purpose, we performed the screening of a collection of keton

SUBMITTER: Ceccoli RD 

PROVIDER: S-EPMC5407406 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature