Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location.

ABSTRACT: Glutamate dehydrogenase is an important enzyme in the hepatic regulation of nitrogen and energy metabolism. It catalyzes one of the most relevant anaplerotic reactions. Although its relevance in liver homeostasis has been widely described, its daily pattern and responsiveness to restricted feeding protocols has not been studied. We explored the daily variations of liver glutamate dehydrogenase transcription, protein, activity, and histochemical and subcellular location in a protocol of daytime food synchronization in rats. Restricted feeding involved food access for 2 h each day for three weeks. Control groups included food ad libitum as well as acute fasting (21 h fasting) and refeeding (22 h fasting followed by 2 h of food access). Glutamate dehydrogenase mRNA, protein, activity, and his

SUBMITTER: Vazquez-Martinez O 

PROVIDER: S-EPMC5407590 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature