Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System.

ABSTRACT: Single-molecule Förster Resonance Energy Transfer (smFRET) can be used to obtain structural information on biomolecular complexes in real-time. Thereby, multiple smFRET measurements are used to localize an unknown dye position inside a protein complex by means of trilateration. In order to obtain quantitative information, the Nano-Positioning System (NPS) uses probabilistic data analysis to combine structural information from X-ray crystallography with single-molecule fluorescence data to calculate not only the most probable position but the complete three-dimensional probability distribution, termed posterior, which indicates the experimental uncertainty. The concept was generalized for the analysis of smFRET networks containing numerous dye molecules. The latest version of NPS, Fast-NPS,

SUBMITTER: Dorfler T 

PROVIDER: S-EPMC5407667 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature