Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. pruni in symptomatic field samples.

ABSTRACT: Xanthomonas arboricola pv. pruni is a quarantine pathogen and the causal agent of the bacterial spot disease of stone fruits and almond, a major threat to Prunus species. Rapid and specific detection methods are essential to improve disease management, and therefore a prototype of a lateral flow immunoassay (LFIA) was designed for the detection of X. arboricola pv. pruni in symptomatic field samples. It was developed by producing polyclonal antibodies which were then combined with carbon nanoparticles and assembled on nitrocellulose strips. The specificity of the LFIA was tested against 87 X. arboricola pv. pruni strains from different countries worldwide, 47 strains of other Xanthomonas species and 14 strains representing other bacterial genera. All X. arboricola pv. pruni strains were de

SUBMITTER: Lopez-Soriano P 

PROVIDER: S-EPMC5407831 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature