Human MicroRNA-548p Decreases Hepatic Apolipoprotein B Secretion and Lipid Synthesis.

ABSTRACT:

Objective

MicroRNAs (miRs) play important regulatory roles in lipid metabolism. Apolipoprotein B (ApoB), as the only essential scaffolding protein in the assembly of very-low-density lipoproteins, is a target to treat hyperlipidemia and atherosclerosis. We aimed to find out miRs that reduce apoB expression.

Approach and results

Bioinformatic analyses predicted that hsa-miR-548p can interact with apoB mRNA. MiR-548p or control miR was transfected in human and mouse liver cells to test its role in regulating apoB secretion and mRNA expression levels. Site-directed mutagenesis was used to identify the interacting site of miR-548p in human apoB 3'-untranslated region. Fatty acid oxidation and lipid syntheses were examined in miR-548p overexpressing cells to investigate its funct

SUBMITTER: Zhou L 

PROVIDER: S-EPMC5408302 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature