Impaired lysosomal activity mediated autophagic flux disruption by graphite carbon nanofibers induce apoptosis in human lung epithelial cells through oxidative stress and energetic impairment.

ABSTRACT:

Background

Graphite carbon nanofibers (GCNF) have emerged as a potential alternative of carbon nanotubes (CNT) for various biomedical applications due to their superior physico-chemical properties. Therefore in-depth understanding of the GCNF induced toxic effects and underlying mechanisms in biological systems is of great interest. Currently, autophagy activation by nanomaterials is recognized as an emerging toxicity mechanism. However, the association of GCNF induced toxicity with this form of cell death is largely unknown. In this study, we have assessed the possible mechanism; especially the role of autophagy, underlying the GCNF induced toxicity.

Methods

Human lung adenocarcinoma (A549) cells were exposed to a range of GCNF concentrations and various cellular parameters

SUBMITTER: Mittal S 

PROVIDER: S-EPMC5408471 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature