Recycler: an algorithm for detecting plasmids from de novo assembly graphs.

ABSTRACT:

Motivation

Plasmids and other mobile elements are central contributors to microbial evolution and genome innovation. Recently, they have been found to have important roles in antibiotic resistance and in affecting production of metabolites used in industrial and agricultural applications. However, their characterization through deep sequencing remains challenging, in spite of rapid drops in cost and throughput increases for sequencing. Here, we attempt to ameliorate this situation by introducing a new circular element assembly algorithm, leveraging assembly graphs provided by a conventional de novo assembler and alignments of paired-end reads to assemble cyclic sequences likely to be plasmids, phages and other circular elements.

Results

We introduce Recycler, the first tool

SUBMITTER: Rozov R 

PROVIDER: S-EPMC5408804 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature