Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.
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ABSTRACT:
SUBMITTER: Bilverstone TW
PROVIDER: S-EPMC5408908 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.
Bilverstone T W TW Kinsmore N L NL Minton N P NP Kuehne S A SA
Anaerobe 20170117
Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity. ...[more]
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