The novel chloroplast outer membrane kinase KOC1 is a required component of the plastid protein import machinery.

ABSTRACT: The biogenesis and maintenance of cell organelles such as mitochondria and chloroplasts require the import of many proteins from the cytosol, a process that is controlled by phosphorylation. In the case of chloroplasts, the import of hundreds of different proteins depends on translocons at the outer and inner chloroplast membrane (TOC and TIC, respectively) complexes. The essential protein TOC159 functions thereby as an import receptor. It has an N-terminal acidic (A-) domain that extends into the cytosol, controls receptor specificity, and is highly phosphorylated in vivo However, kinases that phosphorylate the TOC159 A-domain to enable protein import have remained elusive. Here, using co-purification with TOC159 from Arabidopsis, we discovered a novel component of the chlor

SUBMITTER: Zufferey M 

PROVIDER: S-EPMC5409464 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature