Demethylation of H3K27 Is Essential for the Induction of Direct Cardiac Reprogramming by miR Combo.

ABSTRACT:

Rationale

Direct reprogramming of cardiac fibroblasts to cardiomyocytes has recently emerged as a novel and promising approach to regenerate the injured myocardium. We have previously demonstrated the feasibility of this approach in vitro and in vivo using a combination of 4 microRNAs (miR-1, miR-133, miR-208, and miR-499) that we named miR combo. However, the mechanism of miR combo mediated direct cardiac reprogramming is currently unknown.

Objective

Here, we investigated the possibility that miR combo initiated direct cardiac reprogramming through an epigenetic mechanism.

Methods and results

Using a quantitative polymerase chain reaction array, we found that histone methyltransferases and demethylases that regulate the trimethylation of H3K27 (H3K27me3), an epigenet

SUBMITTER: Dal-Pra S 

PROVIDER: S-EPMC5409871 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature