Transcriptome-wide N ⁶ -methyladenosine methylome profiling of porcine muscle and adipose tissues reveals a potential mechanism for transcriptional regulation and differential methylation pattern.

ABSTRACT:

Background

N ⁶ -methyladenosine (m⁶A) is the most prevalent internal form of modification in messenger RNA in higher eukaryotes and potential regulatory functions of reversible m⁶A methylation on mRNA have been revealed by mapping of m⁶A methylomes in several species. m⁶A modification in active gene regulation manifests itself as altered methylation profiles in a tissue-specific manner or in response to changing cellular or species living environment. However, up to date, there has no data on m⁶A porcine transcriptome-wide map and its potential biological roles in adipose deposition and muscle growth.

Methods

In this work, we used methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-Seq) t

SUBMITTER: Tao X 

PROVIDER: S-EPMC5410061 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature