Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum.

ABSTRACT: The secretory pathway in Plasmodium falciparum has evolved to transport proteins to the host cell membrane and to an endosymbiotic organelle, the apicoplast. The latter can occur via the ER or the ER-Golgi route. Here, we study these three routes using proteins Erythrocyte Membrane Protein-1 (PfEMP1), Acyl Carrier Protein (ACP) and glutathione peroxidase-like thioredoxin peroxidase (PfTPx_Gl) and inhibitors of vesicular transport. As expected, the G protein-dependent vesicular fusion inhibitor AlF_4^- and microtubule destabilizing drug vinblastine block the trafficking of PfEMP-1, a protein secreted to the host cell membrane. However, while both PfTPx_Gl and ACP are targeted to the apicoplast, only ACP trafficking remains unaffected by these t

SUBMITTER: Chaudhari R 

PROVIDER: S-EPMC5410153 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature