CUL-2^LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis.

ABSTRACT: Replisome disassembly is the final step of DNA replication in eukaryotes, involving the ubiquitylation and CDC48-dependent dissolution of the CMG helicase (CDC45-MCM-GINS). Using Caenorhabditis elegans early embryos and Xenopus laevis egg extracts, we show that the E3 ligase CUL-2^LRR-1 associates with the replisome and drives ubiquitylation and disassembly of CMG, together with the CDC-48 cofactors UFD-1 and NPL-4. Removal of CMG from chromatin in frog egg extracts requires CUL2 neddylation, and our data identify chromatin recruitment of CUL2^LRR1 as a key regulated step during DNA replication termination. Interestingly, however, CMG persists on chromatin until prophase in worms that lack CUL-2^LRR-1, but is then removed by a mitotic pathway that requires the

SUBMITTER: Sonneville R 

PROVIDER: S-EPMC5410169 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature