Mutational studies on single circulating tumor cells isolated from the blood of inflammatory breast cancer patients.

ABSTRACT:

Purpose

The molecular characterization of circulating tumor cells (CTCs) is critical to identify the key drivers of cancer metastasis and devising therapeutic approaches, particularly for inflammatory breast cancer (IBC) which is usually diagnosed at advance stages and progresses rapidly.

Methods

Genomic alterations in tumor tissue samples were studied using Foundation One™. Single CTCs were isolated using CellSearch followed by single-cell isolation by DEPArray™. Samples with 20 or more CTCs were chosen to isolate single CTCs using the DEPArray™.

Results

Genomic alterations were studied in primary tumor or metastatic sites from 32 IBC patients. Genes with high-frequency mutations were as follows: TP53 (69%), RB1 (16%), PIK3CA (13%), and also ErbB2 (3%). At least once

SUBMITTER: Bingham C 

PROVIDER: S-EPMC5410214 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature