Improving Focal Photostimulation of Cortical Neurons with Pre-derived Wavefront Correction.

ABSTRACT: Recent progress in neuroscience to image and investigate brain function has been made possible by impressive developments in optogenetic and opto-molecular tools. Such research requires advances in optical techniques for the delivery of light through brain tissue with high spatial resolution. The tissue causes distortions to the wavefront of the incoming light which broadens the focus and consequently reduces the intensity and degrades the resolution. Such effects are detrimental in techniques requiring focal stimulation. Adaptive wavefront correction has been demonstrated to compensate for these distortions. However, iterative derivation of the corrective wavefront introduces time constraints that limit its applicability to probe living cells. Here, we demonstrate that we can pre-determin

SUBMITTER: Choy JMC 

PROVIDER: S-EPMC5410561 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature