The B₁₂-Radical SAM Enzyme PoyC Catalyzes Valine C_β-Methylation during Polytheonamide Biosynthesis.

ABSTRACT: Genomic and metagenomic investigations have recently led to the delineation of a novel class of natural products called ribosomally synthesized and post-translationally modified peptides (RiPPs). RiPPs are ubiquitous among living organisms and include pharmaceutically relevant compounds such as antibiotics and toxins. A prominent example is polytheonamide A, which exhibits numerous post-translational modifications, some of which were unknown in ribosomal peptides until recently. Among these post-translational modifications, C-methylations have been proposed to be catalyzed by two putative radical S-adenosylmethionine (rSAM) enzymes, PoyB and PoyC. Here we report the in vitro activity of PoyC, the first B₁₂-dependent rSAM enzyme catalyzing peptide C_β-methylation. We sh

SUBMITTER: Parent A 

PROVIDER: S-EPMC5410653 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature