Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression.

ABSTRACT: The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodule-specific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes' active or repressed state. On the other hand, expression of nodule-specific genes correlated with plo

SUBMITTER: Nagymihaly M 

PROVIDER: S-EPMC5410778 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature