Conserved effect of aging on DNA methylation and association with EZH2 polycomb protein in mice and humans.

ABSTRACT: In humans, DNA methylation at specific CpG sites can be used to estimate the 'epigenetic clock', a biomarker of aging and health. The mechanisms that regulate the aging epigenome and level of conservation are not entirely clear. We performed affinity-based enrichment with methyl-CpG binding domain protein followed by high-throughput sequencing (MBD-seq) to assay DNA methylation in mouse samples. Consistent with previous reports, aging is associated with increase in methylation at CpG islands that likely overlap regulatory regions of genes that have been implicated in cancers (e.g., C1ql3, Srd5a2 and Ptk7). The differentially methylated regions in mice have high sequence conservation in humans and the pattern of methylation is also largely conserved between the two species. Based on human E

SUBMITTER: Mozhui K 

PROVIDER: S-EPMC5411177 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature