Transcriptional signatures throughout development: the effects of mouse embryo manipulation in vitro.

ABSTRACT: Stressful environmental exposures incurred early in development can affect postnatal metabolic health and susceptibility to non-communicable diseases in adulthood, although the molecular mechanisms by which this occurs have yet to be elucidated. Here, we use a mouse model to investigate how assorted in vitro exposures restricted exclusively to the preimplantation period affect transcription both acutely in embryos and long term in subsequent offspring adult tissues, to determine if reliable transcriptional markers of in vitro stress are present at specific developmental time points and throughout development. Each in vitro fertilization or embryo culture environment led to a specific and unique blastocyst transcriptional profile, but we identified a common 18-gene and 9-pathway signature o

SUBMITTER: Feuer SK 

PROVIDER: S-EPMC5411347 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature