SAT2 Foot-and-Mouth Disease Virus Structurally Modified for Increased Thermostability.

ABSTRACT: Foot-and-mouth disease virus (FMDV), particularly strains of the O and SAT serotypes, is notoriously unstable. Consequently, vaccines derived from heat-labile SAT viruses have been linked to the induction of immunity with a poor duration and hence require more frequent vaccinations to ensure protection. In silico calculations predicted residue substitutions that would increase interactions at the interpentamer interface, supporting increased stability. We assessed the stability of the 18 recombinant mutant viruses in regard to their growth kinetics, antigenicity, plaque morphology, genetic stability, and temperature, ionic, and pH stability by using Thermofluor and inactivation assays in order to evaluate potential SAT2 vaccine candidates with improved stability. The most stable mut

SUBMITTER: Scott KA 

PROVIDER: S-EPMC5411616 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature