Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster.

ABSTRACT: Highly repetitive satellite DNA (satDNA) repeats are found in most eukaryotic genomes. SatDNAs are rapidly evolving and have roles in genome stability and chromosome segregation. Their repetitive nature poses a challenge for genome assembly and makes progress on the detailed study of satDNA structure difficult. Here, we use single-molecule sequencing long reads from Pacific Biosciences (PacBio) to determine the detailed structure of all major autosomal complex satDNA loci in Drosophila melanogaster, with a particular focus on the 260-bp and Responder satellites. We determine the optimal de novo assembly methods and parameter combinations required to produce a high-quality assembly of these previously unassembled satDNA loci and validate this assembly using molecular an

SUBMITTER: Khost DE 

PROVIDER: S-EPMC5411766 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature