MicroRNA-223 demonstrated experimentally in exosome-like vesicles is associated with decreased risk of persistent pain after lumbar disc herniation.

ABSTRACT:

Background

Previous findings have demonstrated that lumbar radicular pain after disc herniation may be associated with up-regulation of inflammatory mediators. In the present study we examined the possible role of extracellular microRNAs (miRs) in this process.

Methods

Single unit recordings, isolation of exosome-like vesicles, electron microscopy, nanoparticle tracking analysis, western blot analysis and qPCR were used in rats to demonstrate the effect of nucleus pulposus (NP) applied onto the dorsal nerve roots. ELISA and qPCR were used to measure the level of circulating IL-6 and miRs in a 1-year observational study in patients after disc herniation.

Results

In the rats, enhanced spinal cord nociceptive responses were displayed after NP applied onto the dorsal nerv

SUBMITTER: Moen A 

PROVIDER: S-EPMC5412060 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature