Both R-loop removal and ribonucleotide excision repair activities of RNase H2 contribute substantially to chromosome stability.

ABSTRACT: Cells carrying deletions of genes encoding H-class ribonucleases display elevated rates of chromosome instability. The role of these enzymes is to remove RNA-DNA associations including persistent mRNA-DNA hybrids (R-loops) formed during transcription, and ribonucleotides incorporated into DNA during replication. RNases H1 and H2 can degrade the RNA component of R-loops, but only RNase H2 can initiate accurate ribonucleotide excision repair (RER). In order to examine the specific contributions of these activities to chromosome stability, we measured rates of loss-of-heterozygosity (LOH) in diploid Saccharomyces cerevisiae yeast strains carrying the rnh201-RED separation-of-function allele, encoding a version of RNase H2 that is RER-defective, but partly retains its other activity. The LOH r

SUBMITTER: Cornelio DA 

PROVIDER: S-EPMC5412515 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature