Time-Course Transcriptome Analysis of Compatible and Incompatible Pollen-Stigma Interactions in Brassica napus L.

ABSTRACT: Brassica species exhibit both compatible and incompatible pollen-stigma interactions, however, the underlying molecular mechanisms remain largely unknown. Here, RNA-seq technology was applied in a comprehensive time-course experiment (2, 5, 10, 20, and 30 min) to explore gene expression during compatible/incompatible pollen-stigma interactions in stigma. Moderate changes of gene expression were observed both in compatible pollination (PC) and incompatible pollination (PI) within 10 min, whereas drastic changes showed up by 30 min, especially in PI. Stage specific DEGs [Differentially Expressed Gene(s)] were identified, and signaling pathways such as stress response, defense response, cell wall modification and others were found to be over-represented. In addition, enriched genes in

SUBMITTER: Zhang T 

PROVIDER: S-EPMC5413569 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature