Mechanism of resonant electron emission from the deprotonated GFP chromophore and its biomimetics.

ABSTRACT: The Green Fluorescent Protein (GFP), which is widely used in bioimaging, is known to undergo light-induced redox transformations. Electron transfer is thought to occur resonantly through excited states of its chromophore; however, a detailed understanding of the electron gateway states of the chromophore is still missing. Here, we use photoelectron spectroscopy and high-level quantum chemistry calculations to show that following UV excitation, the ultrafast electron dynamics in the chromophore anion proceeds via an excited shape resonance strongly coupled to the open continuum. The impact of this state is found across the entire 355-315 nm excitation range, from above the first bound-bound transition to below the opening of higher-lying continua. By disentangling the electron dynami

SUBMITTER: Bochenkova AV 

PROVIDER: S-EPMC5413970 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature