Dataset Information

Circulating DNA in rheumatoid arthritis: pathological changes and association with clinically used serological markers.

ABSTRACT:

Background

Early diagnosis of rheumatoid arthritis (RA) is crucial to providing effective therapy and often hampered by unspecific clinical manifestations. Elevated levels of extracellular circulating DNA (cirDNA) in patients with autoimmune disease were found to be associated with etiopathogenesis. To our knowledge, this is the first study to investigate the putative diagnostic use of cirDNA in RA and its association with disease activity.

Methods

Blood samples were taken from 63 healthy subjects (HS) and 74 patients with RA. cirDNA was extracted from plasma and cell surface-bound cirDNA fractions (csbDNA). cirDNA concentration was measured by quantitative real-time polymerase chain reaction. Rheumatoid factor was analyzed by immunonephelometry, whereas C-reactive protein and anticitrullinated protein/peptide antibodies (ACPA) were detected by enzyme-linked immunosorbent assay.

Results

Plasma cirDNA was significantly elevated in patients with RA compared with HS (12.0 versus 8.4 ng/ml, p < 0.01). In contrast, nuclear csbDNA (n-csbDNA) was significantly decreased (24.0 versus 50.8 ng/ml, p < 0.01), whereas mitochondrial csbDNA (m-csbDNA) was elevated (1.44 × 10⁶ copies/ml versus 0.58 × 10⁶ copies/ml, p < 0.05) in RA. The combination of csbDNA (mitochondrial + nuclear) with ACPA reveals the best positive/negative likelihood ratios (LRs) for the discrimination RA from HS (LR+ 61.00, LR- 0.03) in contrast to ACPA (LR+ 9.00, LR- 0.19) or csbDNA (LR+ 8.00, LR- 0.18) alone.

Conclusions

Nuclear and mitochondrial cirDNA levels in plasma and on the surface of blood cells are modulated in RA. Combination of cirDNA values with ACPA can improve the serological diagnosis of RA.

SUBMITTER: Rykova E

PROVIDER: S-EPMC5414163 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Publications

Circulating DNA in rheumatoid arthritis: pathological changes and association with clinically used serological markers.

Rykova Elena E Sizikov Aleksey A Roggenbuck Dirk D Antonenko Oksana O Bryzgalov Leonid L Morozkin Evgeniy E Skvortsova Kseniya K Vlassov Valentin V Laktionov Pavel P Kozlov Vladimir V

Arthritis research & therapy 20170502 1

<h4>Background</h4>Early diagnosis of rheumatoid arthritis (RA) is crucial to providing effective therapy and often hampered by unspecific clinical manifestations. Elevated levels of extracellular circulating DNA (cirDNA) in patients with autoimmune disease were found to be associated with etiopathogenesis. To our knowledge, this is the first study to investigate the putative diagnostic use of cirDNA in RA and its association with disease activity.<h4>Methods</h4>Blood samples were taken from 63 ...[more]

PMID: 28464939

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Dataset Information

Circulating DNA in rheumatoid arthritis: pathological changes and association with clinically used serological markers.

Background

Methods

Results

Conclusions

Publications

Circulating DNA in rheumatoid arthritis: pathological changes and association with clinically used serological markers.

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