Purification and characterization of a novel cold adapted fungal glucoamylase.

ABSTRACT:

Background

Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For example, the most commonly used industrial glucoamylases, a type of amylase that degrades starch to glucose, are produced by Aspergillus strains displaying optimal activities at 45-60 °C. Thus, isolating new amylases with optimal activity at ambient temperature is essential for improving industrial processes. In this report, a glucoamylase secreted by the cold-adapted yeast Tetracladium sp. was isolated and biochemically characterized.

Results

The effects of ph

SUBMITTER: Carrasco M 

PROVIDER: S-EPMC5414198 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature