Geno- and phenotypic characteristics of a transfected Babesia bovis 6-Cys-E knockout clonal line.

ABSTRACT:

Background

Babesia bovis is an intra-erythrocytic tick-transmitted apicomplexan protozoan parasite. It has a complex lifestyle including asexual replication in the mammalian host and sexual replication occurring in the midgut of host tick vector, typically, Rhipicephalus microplus. Previous evidence showed that certain B. bovis genes, including members of 6-Cys gene family, are differentially expressed during tick and mammalian stages of the parasite's life cycle. Moreover, the 6-Cys E gene is differentially expressed in the T3Bo strain of B. bovis tick stages, and anti 6-Cys E antibodies were shown to be able to inhibit in vitro growth of the phenotypically distinct B. bovis Mo7clonal line.

Methods

In this study, the 6-Cys E gene of B. bovis T3Bo strain was disrupted by tra

SUBMITTER: Alzan HF 

PROVIDER: S-EPMC5414359 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature