The development of sexual stage malaria gametocytes in a Wave Bioreactor.

ABSTRACT:

Background

Blocking malaria gametocyte development in RBCs or their fertilization in the mosquito gut can prevent infection of the mosquito vector and passage of disease to the human host. A 'transmission blocking' strategy is a component of future malaria control. However, the lack of robust culture systems for producing large amounts of Plasmodium falciparum gametocytes has limited our understanding of sexual-stage malaria biology and made vaccine or chemotherapeutic discoveries more difficult.

Methods

The Wave Bioreactor^TM 20/50 EHT culture system was used to develop a convenient and low-maintenance protocol for inducing commitment of P. falciparum parasites to gametocytogenesis. Culture conditions were optimised to obtain mature stage V gametocytes within 2 we

SUBMITTER: Demanga CG 

PROVIDER: S-EPMC5414375 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature