Research resource: the dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis.

ABSTRACT: Sertoli cells (SCs), the only somatic cells within seminiferous tubules, associate intimately with developing germ cells. They not only provide physical and nutritional support but also secrete factors essential to the complex developmental processes of germ cell proliferation and differentiation. The SC transcriptome must therefore adapt rapidly during the different stages of spermatogenesis. We report comprehensive genome-wide expression profiles of pure populations of SCs isolated at 5 distinct stages of the first wave of mouse spermatogenesis, using RNA sequencing technology. We were able to reconstruct about 13 901 high-confidence, nonredundant coding and noncoding transcripts, characterized by complex alternative splicing patterns with more than 45% comprising novel isoforms of known

SUBMITTER: Zimmermann C 

PROVIDER: S-EPMC5414748 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature