Human cytomegalovirus glycoprotein polymorphisms and increasing viral load in AIDS patients.

ABSTRACT:

Background

Multiple strains infection of human cytomegalovirus (HCMV) was found to be correlated with increased viral load in immunodeficient patients. However, the pathogenic mechanism underlying this correlation remains unclear. To evaluate genetic polymorphisms of HCMV glycoprotein and their potential role in its viral load, HCMV glycoprotein B, N, and O (gB, gN and gO) genotypes was studied in the population of HCMV infected acquired immune deficiency syndrome (AIDS) patients. The association between glycoprotein polymorphisms and HCMV viral load was analyzed.

Methods

The genetic polymorphisms of glycoprotein from sera of 60 HCMV infected AIDS patients was investigated by multiplex nested PCR and sequencing. HCMV viral load was evaluated by quantitative PCR.

Results</

SUBMITTER: Jiang XJ 

PROVIDER: S-EPMC5415198 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature