Research resource: small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes.

ABSTRACT: The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signaling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and in vitro fertilization success. However, the posttranscriptional gene expression studies on micro-RNA (miRNA) level in the human ovary have been scarce. The current study determined the miRNA profile by deep sequencing of the 2 intrafollicular somatic cell types: mural and cumulus granulosa cells (MGCs and CGCs, respectively) isolated from women undergoing controlled ovarian stimulation and in vitro fertilization. Altogether, 936 annotated and 9 novel miRNAs were identified. Ninety of

SUBMITTER: Velthut-Meikas A 

PROVIDER: S-EPMC5415242 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature