Epithelial ciliated beating cells essential for ex vivo ALI culture growth.

ABSTRACT:

Background

Bronchial epithelium plays a key role in orchestrating innate and adaptive immunity. The fate of ex vivo airway epithelial cultures growing at the air liquid interface (ALI) derived from human endobronchial biopsies or brushings is not easy to predict. Calibrating and differentiating these cells is a long and expensive process requiring rigorous expertise. Pinpointing factors associated with ALI culture success would help researchers gain further insight into epithelial progenitor behavior.

Methods

A successful ALI culture was defined as one in which a pseudostratified epithelium has formed after 28 days in the presence of all differentiated epithelial cell types. A 4-year prospective bi-center study was conducted with adult subjects enrolled in different approved

SUBMITTER: Gras D 

PROVIDER: S-EPMC5415749 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature