Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneo.

ABSTRACT:

Background

How nutrition and growth factor restriction due to serum depletion affect trophoblast function remains poorly understood. We performed a proteomic differential study of the effects of serum depletion on a first trimester human immortalized trophoblast cell line.

Methods

The viability of HTR-8/SVneo trophoblast cells in culture with 0, 0.5 and 10 % fetal bovine serum (FBS) were assayed via MTT at 24, 48 and 64 h. A comparative proteomic analysis of the cells grown with those FBS levels for 24 h was performed using two-dimensional electrophoresis (2DE), followed by mass spectrometry for protein spot identification, and a database search and bioinformatics analysis of the expressed proteins. Differential spots were identified using the Kolmogorov-Smirnov test (n</

SUBMITTER: Novoa-Herran S 

PROVIDER: S-EPMC5415790 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature