Effect of differentiation on microRNA expression in bovine skeletal muscle satellite cells by deep sequencing.

ABSTRACT:

Background

The differentiation of skeletal muscle-derived satellite cells (MDSCs) is important in controlling muscle growth, improving livestock muscle quality, and healing of muscle-related disease. MicroRNAs (miRNAs) are a class of gene expression regulatory factors, which play critical roles in the regulation of muscle cell differentiation. This study aimed to compare the expression profile of miRNAs in MDSC differentiation, and to investigate the miRNAs which are involved in MDSC differentiation.

Method

Total RNA was extracted from MDSCs at three different stages of differentiation (MDSC-P, MDSC-D1 and MDSC-D3, representing 0, 1 and 3 days after differentiation, respectively), and used to construct small RNA libraries for RNA sequencing (RNA-seq).

Results

The resu

SUBMITTER: Zhang WW 

PROVIDER: S-EPMC5415838 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature