Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification.

ABSTRACT: Cre/LoxP technology is widely used in the field of mouse genetics for spatial and/or temporal regulation of gene function. For Cre lines generated via pronuclear microinjection of a Cre transgene construct, the integration site is random and in most cases not known. Integration of a transgene can disrupt an endogenous gene, potentially interfering with interpretation of the phenotype. In addition, knowledge of where the transgene is integrated is important for planning of crosses between animals carrying a conditional allele and a given Cre allele in case the alleles are on the same chromosome. We have used targeted locus amplification (TLA) to efficiently map the transgene location in seven previously published Cre and CreERT2 transgenic lines. In all lines, transgene insertion was associ

SUBMITTER: Cain-Hom C 

PROVIDER: S-EPMC5416772 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature